Selective Protein Capture by Epitope Imprinting

Angew. Chem. Int. Ed. 2006, 45, 2392—2396

A general protocol for creating macromolecular receptors for proteins has been developed. It utilizes the sequences of exposed epitopes based on known or predicted protein structures. This method requires only the peptide sequence of a small portion of the target protein for the template molecule. If is bases on the premise that capture of the epitope is all that is necessary to capture the whole protein.

Protocol for template imprinting with protein epitopes. A) Method for glass modification and peptide attachment. The peptide epitopes were attached to the glass surface. B) Illustration outlining MIP film fabrication and evaluation. Monomers are photochemically cross-linked with a UV lamp . Following polymerization, the glass substrate and/or silicon wafer was removed by soaking in buffer C) Proposed mechanism for the target protein recognition of Cterminus peptide-sequence-imprinted surfaces. A nanocavity-bound target protein is caused by many cooperative weak interactions that involve hydrogen bonds and hydrophobic interactions.